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Tuesday, May 19, 2020 | History

8 edition of PRINS and in situ PCR protocols found in the catalog.

PRINS and in situ PCR protocols

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Published by Humana Press in Totowa, N.J .
Written in English

    Subjects:
  • In situ hybridization -- Laboratory manuals.,
  • Polymerase chain reaction -- Laboratory manuals.,
  • In Situ Hybridization -- methods -- laboratory manuals.,
  • DNA Primers -- chemistry -- laboratory manuals.,
  • Polymerase Chain Reaction -- methods -- laboratory manuals.,
  • Sequence Analysis -- methods -- laboratory manuals.,
  • Chromosome Mapping -- methods -- laboratory manuals.

  • Edition Notes

    Includes bibliographical references and index.

    Statementedited by John R. Gosden.
    SeriesMethods in molecular biology ;, v. 71, Methods in molecular biology (Clifton, N.J.) ;, v. 71.
    ContributionsGosden, John R.
    Classifications
    LC ClassificationsQH452.8 .P75 1997
    The Physical Object
    Paginationxiii, 165 p. :
    Number of Pages165
    ID Numbers
    Open LibraryOL994220M
    ISBN 100896033953
    LC Control Number96032765

    Primed in situ labeling (PRINS) is a fast and sensitive technique for sequence specific in situ detection of DNA. An unlabeled oligonucleotide probe is hybridized and used as primer for chain elongation in situ catalyzed by a DNA polymerase. Thus, the oligonucleotide primer binds by sequence-specific base pairing to its target sequence, which is subsequently labeled when Cited by: 1.   PRINS and In Situ PCR Protocols The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic : Min Li.

    This book describes the methodology developed for the in situ detection of PCR-amplified DNA and cDNA. Applications of PCR in situ hybridization and reverse transcription in situ PCR are given for the study of infection by several human infectious agents, including the human immunodeficiency virus The basics of the various techniques are also by: PRINS and In Situ PCR Protocols: Second Edition, edited by Franck Pellestor, Transplantation Immunology: Methods and Protocols, edited by Philip Hornick and Marlene Rose, Transmembrane Signaling Protocols: Second Edition, edited by Hydar Ali and Bodduluri Haribabu, Human Embryonic Stem Cell Protocols, edited.

    different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cultures were used to obtain metaphases from male and female : Paulo Z. Passamani, Carlos R. Carvalho, Fernanda A.F. Soares. In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random Vitro Mutagenesis: Methods and Protocols .


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PRINS and in situ PCR protocols Download PDF EPUB FB2

Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling : Hardcover.

Buy PRINS and In Situ PCR Protocols (Methods in Molecular Biology) on FREE SHIPPING on qualified orders PRINS and In Situ PCR Protocols (Methods in Molecular Biology): Franck Pellestor: : Books.

Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology. PRINS and In Situ PCR Protocols. Editors (view affiliations) John R.

Gosden; Book. 11 Citations; k Downloads; Part of the Methods in Molecular Biology™ book series (MIMB, volume 71) Protocols Table of contents (16 protocols) About About this book; Table of contents.

Search within book. PRINS and in situ PCR protocols by Gosden, John R. Publication date Topics In situ hybridization, Polymerase chain reaction, In Situ Hybridization, DNA Primers, Polymerase Chain Reaction, Sequence Analysis, Chromosome Mapping Publisher Internet Archive Books.

Scanned in : In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions.

This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure.

Table of contents for PRINS and in situ PCR protocols. Bibliographic record and links to related information available from the Library of Congress catalog. Note: Contents data are machine generated based on pre-publication provided by the publisher.

Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by : J J O'Leary. This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS.

Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS. In Situ PCR and PRINS PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues: Technical Considerations on PRINS and In Situ PCR, and Comparison with In Situ Hybridization Ernst J.

Speel, Frans C. Ramaekers, and Anton H. Hopman Cycling Primed In Situ Amplification John H. Bull and Lynn Paskins Direct and Indirect In. This completely revised and updated second edition presents a comprehensive selection of new procedures developed in the field of PRINS and in situ PCR technologies.

The first section on basis methodology contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocol for PRINS detection of unique sequences in situ.

Summary: The techniques of oligonucleotide-primed in situ synthesis (PRINS) and in situ PCR are growing rapidly. This text intends to serve as a foundation stone and catalyst for future developments in the field. The protocols are written in a step-by-step format and are supported with a section of notes.

Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a page image below to browse page by page. In the first edition of PRINS and In Situ PCR Protocols edited by John R.

Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in. PRINS and in-situ PCR Protocols. By J J O'Leary. Topics: Book Reviews. Year: OAI identifier: oai: Provided by: PubMed Central.

Description of the dataset: Open Access subset. Suggested articles. If you think this Author: J J O'Leary. In situ PCR has been described and performed extensively in animals and a detailed protocol has been made available [15]. Reports of in situ PCR on plant material either include lengthy tissue preparations, thermal cycling of slide-mounted specimens, or a detailed protocol was not supplied [].Cited by: Here, we describe real-time PCR protocols suitable for relative and absolute quantification of TYLCSV in tomato plants and in whitefly extracts.

Using primers and probe specifically designed. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions.

Since the publication of this successful reference book, there have been sig-nificant improvements in in situ detection. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization.

Color illustrations enhance the text.This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant.

Main PCR Protocols. PCR Protocols John M. S. Bartlett, David as well as in-depth insight into a variety of the more complex applications of PCR.

This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work. prins chain reaction sample.